villaines: meaning either servants, peasants or wrongdoers

Are of consent and sufferance in this: are accessories who have allowed this to happen; have approved this and made it possible

a bed: in bed

untreasur'd: robbed

roynish: contemptible, mangy, scurvy

wont: accustomed

Hisperia: Was properly one of the "Hesperides", the nymphs set to guard the tree that bore golden apples which was given by Jupiter to Juno as a wedding gift. Hercules managed to steal some of the golden apples in his eleventh labor, so this Hisperia, fails in her duty.

parts and graces: qualities, physical characteristics and abilities

Send this to his brother: Duke Frederick asks that they send a message to Orlando's brother, Oliver, who would have the authority to "fetch that gallant", who is Orlando. If he's absent, he asks that Oliver be brought in his stead.

gallant: fine gentlemen (he speaks sarcastically of Orlando)

inquisition: questioning

quaile: falter